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simplechip plus enzymatic chromatin ip kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc simplechip plus enzymatic chromatin ip kit
    Simplechip Plus Enzymatic Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simplechip+plus+enzymatic+chromatin+ip/pm41945357-63-5-11
    Average 86 stars, based on 1 article reviews
    simplechip plus enzymatic chromatin ip kit - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Replacing reprogramming factors with antibodies selected from combinatorial antibody libraries.
    Article Snippet: The membranes were blocked with 5% milk in TBST for 2 h and were immunoblotted with p-Smad2/3 (1:1,000, Cell Signaling #8828) and detected with anti-rabbit IgG HRP (1:3,000, Cell Signaling, #7074). .. Chromatin immunoprecipitation was performed using SimpleChip Plus Enzymatic Chromatin IP (Cell Signaling) starting with 2 μg input DNA. ..

    Article Title: Gene repression through epigenetic modulation by PPARA enhances hepatocellular proliferation
    Article Snippet: .. SimpleChIP Plus Enzymatic Chromatin IP , Cell Signaling Technology , Cat# 9005. .. ALT assay kit , Catachem Inc , Cat# V164.

    Article Title: MicroRNA-148a facilitates inflammatory dendritic cell differentiation and autoimmunity by targeting MAFB
    Article Snippet: .. ChIP assays were conducted with a SimpleChIP Plus Enzymatic Chromatin IP (Magnetic Beads) Kit (Cell Signaling Technology). ..

    Article Title: Replacing reprogramming factors with antibodies selected from autocrine antibody libraries
    Article Snippet: The membranes were blocked with 5% milk in TBST for 2 hours and were immunoblotted with p-Smad 2/3 (1:1000, Cell Signaling #8828) and detected with anti-rabbit IgG HRP (1:3000, Cell Signaling, #7074). .. Chromatin immunopercipitations were performed using SimpleChip Plus Enzymatic Chromatin IP (Cell signaling) starting with 2 μg of input DNA. ..

    Article Title: The signal transducer CD24 suppresses the germ cell program and promotes an ectodermal rather than mesodermal cell fate in embryonal carcinomas.
    Article Snippet: Briefly, 1 9 107 cells were fixed for 30 min at RT using Diagenode ‘Crosslink Gold’ Kit (Diagenode). .. Cells were fixed 10 min in 1% formaldehyde (AppliChem, Darmstadt, Germany) and processed according to the ‘SimpleChIP Plus Enzymatic Chromatin IP’ Kit (Cell Signaling Technology, Leiden, The Netherlands). ..

    Article Title: The signal transducer CD24 suppresses the germ cell program and promotes an ectodermal rather than mesodermal cell fate in embryonal carcinomas
    Article Snippet: Briefly, 1 × 10 7 cells were fixed for 30 min at RT using Diagenode ‘Crosslink Gold’ Kit (Diagenode). .. Cells were fixed 10 min in 1% formaldehyde (AppliChem, Darmstadt, Germany) and processed according to the ‘SimpleChIP Plus Enzymatic Chromatin IP’ Kit (Cell Signaling Technology, Leiden, The Netherlands). ..

    Magnetic Beads:

    Article Title: MicroRNA-148a facilitates inflammatory dendritic cell differentiation and autoimmunity by targeting MAFB
    Article Snippet: .. ChIP assays were conducted with a SimpleChIP Plus Enzymatic Chromatin IP (Magnetic Beads) Kit (Cell Signaling Technology). ..



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    simplechip plus enzymatic chromatin ip kit  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc simplechip plus enzymatic chromatin ip kit
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    https://www.bioz.com/product/simplechip+plus+enzymatic+chromatin+ip/pm41945357-63-5-11
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    chip kit  (Cell Signaling Technology Inc)
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    <t>YAP</t> directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) <t>ChIP-qPCR</t> analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.
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    enzymatic chromatin ip assay  (Cell Signaling Technology Inc)
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    <t>YAP</t> directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) <t>ChIP-qPCR</t> analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.
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    chip plus enzymatic chromatin ip kit  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc chip plus enzymatic chromatin ip kit
    <t>YAP</t> directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) <t>ChIP-qPCR</t> analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.
    Chip Plus Enzymatic Chromatin Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simplechip+plus+enzymatic+chromatin+ip/SimpleChIP+Enzymatic+Chromatin+IP+Kit/pmc13023030-36-6-13
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    Image Search Results


    YAP directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) ChIP-qPCR analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.

    Journal: Redox Biology

    Article Title: NMRK2–YAP–NADK axis preserves redox protection against myocardial ischemia/reperfusion injury

    doi: 10.1016/j.redox.2026.104100

    Figure Lengend Snippet: YAP directly binds to the NADK promoter and activates its transcription. (A) Schematic illustration of the human NADK promoter region (−2000 to −0 bp relative to the transcription start site) showing putative YAP-binding sites and the design of a series of truncated NADK promoter luciferase reporter constructs. (B) ChIP-qPCR analysis demonstrating significant enrichment of the NADK promoter region in anti-YAP immunoprecipitates compared with IgG controls ( n = 3). (C) Luciferase reporter assays showing that YAP overexpression significantly enhances transcriptional activity driven by the −2000/0 and −1500/0 NADK promoter fragments, whereas further truncation to −1000/0 or −500/0 markedly attenuates YAP-induced activation, identifying the YAP-responsive region within the −1500 to −1000 bp interval ( n = 3). Data are presented as mean ± SEM. Unpaired Student's t -test or one-way ANOVA followed by Tukey's post hoc test for comparisons. *** P < 0.001. ChIP, Chromatin immunoprecipitation; IgG, immunoglobulin G; LUC, luciferase; Rluc, Renilla luciferase; NC, negative control.

    Article Snippet: ChIP assays were performed using a commercial ChIP kit (CST 9005S) to assess the binding of YAP to the NADK promoter.

    Techniques: Binding Assay, Luciferase, Construct, ChIP-qPCR, Over Expression, Activity Assay, Activation Assay, Chromatin Immunoprecipitation, Negative Control